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SHIMADA TOMOHIRO
Department Undergraduate School , School of Agriculture Position Associate Professor |
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| Language | English |
| Publication Date | 2014/07 |
| Type | Academic Journal |
| Peer Review | Peer reviewed |
| Title | Unprecedented high-resolution view of bacterial operon architecture revealed by RNA sequencing. |
| Contribution Type | Co-authored (other than first author) |
| Journal | mBio |
| Journal Type | Another Country |
| Volume, Issue, Page | 5(4),pp.e01442-14 |
| International coauthorship | International coauthorship |
| Author and coauthor | Conway Tyrrell, Creecy James P, Maddox Scott M, Grissom Joe E, Conkle Trevor L, Shadid Tyler M, Teramoto Jun, San Miguel Phillip, Shimada Tomohiro, Ishihama Akira, Mori Hirotada, Wanner Barry L |
| Details | We analyzed the transcriptome of Escherichia coli K-12 by strand-specific RNA sequencing at single-nucleotide resolution during steady-state (logarithmic-phase) growth and upon entry into stationary phase in glucose minimal medium. To generate high-resolution transcriptome maps, we developed an organizational schema which showed that in practice only three features are required to define operon architecture: the promoter, terminator, and deep RNA sequence read coverage. We precisely annotated 2,122 promoters and 1,774 terminators, defining 1,510 operons with an average of 1.98 genes per operon. ... |
| DOI | 10.1128/mBio.01442-14 |
| ISSN | 2150-7511 |
| PMID | 25006232 |