|
SHIMADA TOMOHIRO
Department Undergraduate School , School of Agriculture Position Associate Professor |
|
| Language | English |
| Publication Date | 2017/12 |
| Type | Academic Journal |
| Peer Review | Peer reviewed |
| Title | Regulatory role of XynR (YagI) in catabolism of xylonate in Escherichia coli K-12. |
| Contribution Type | Co-authored (first author) |
| Journal | FEMS microbiology letters |
| Journal Type | Another Country |
| Volume, Issue, Page | 364(22) |
| Authorship | Lead author |
| Author and coauthor | Shimada Tomohiro, Momiyama Eri, Yamanaka Yuki, Watanabe Hiroki, Yamamoto Kaneyoshi, Ishihama Akira |
| Details | The genome of Escherichia coli K-12 contains ten cryptic phages, altogether constituting about 3.6% of the genome in sequence. Among more than 200 predicted genes in these cryptic phages, 14 putative transcription factor (TF) genes exist, but their regulatory functions remain unidentified. As an initial attempt to make a breakthrough for understanding the regulatory roles of cryptic phage-encoded TFs, we tried to identify the regulatory function of CP4-6 cryptic prophage-encoded YagI with unknown function. After SELEX screening, YagI was found to bind mainly at a single site within the spacer of bidirectional transcription units, yagA (encoding another uncharacterized TF) and yagEF (encoding 2-keto-3-deoxy gluconate aldolase, and dehydratase, respectively) within this prophage region. ... |
| DOI | 10.1093/femsle/fnx220 |
| ISSN | 1574-6968 |
| PMID | 29087459 |