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SHIMADA TOMOHIRO
Department Undergraduate School , School of Agriculture Position Associate Professor |
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| Language | English |
| Publication Date | 2022/04 |
| Type | Academic Journal |
| Title | Regulatory role of CsuR (YiaU) in determination of cell surface properties of Escherichia coli K-12. |
| Contribution Type | Co-authored (first author) |
| Journal | Microbiology (Reading, England) |
| Journal Type | Another Country |
| Volume, Issue, Page | 168(4) |
| Authorship | Corresponding author |
| Author and coauthor | Tomohiro Shimada, Rie Murayama, Tomoki Mashima, Natsuko Kawano, Akira Ishihama |
| Details | Genomic SELEX screening was performed to identify the binding sites of YiaU, an uncharacterized LysR family transcription factor, on the Escherichia coli K-12 genome. Five high-affinity binding targets of YiaU were identified, all of which were involved in the structures of the bacterial cell surface such as outer and inner membrane proteins, and lipopolysaccharides. Detailed in vitro and in vivo analyses suggest that YiaU activates these target genes. To gain insight into the effects of YiaU in vivo on physiological properties, we used phenotype microarrays, biofilm screening assays and the sensitivity against serum complement analysed using a yiaU deletion mutant or YiaU expression strain. Together, these results suggest that the YiaU regulon confers resistance to some antibiotics, and increases biofilm formation and complement sensitivity. We propose renaming YiaU as CsuR (regulator of cell surface). |
| DOI | 10.1099/mic.0.001166 |
| PMID | 35438626 |