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SHIMADA TOMOHIRO
Department Undergraduate School , School of Agriculture Position Associate Professor |
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| Language | English |
| Publication Date | 2008/09 |
| Type | Academic Journal |
| Peer Review | Peer reviewed |
| Title | The uncharacterized transcription factor YdhM is the regulator of the nemA gene, encoding N-ethylmaleimide reductase. |
| Contribution Type | Co-authored (other than first author) |
| Journal | Journal of bacteriology |
| Journal Type | Another Country |
| Volume, Issue, Page | 190(17),pp.5890-7 |
| Author and coauthor | Umezawa Yoshimasa, Shimada Tomohiro, Kori Ayako, Yamada Kayoko, Ishihama Akira |
| Details | N-ethylmaleimide (NEM) has been used as a specific reagent of Cys modification in proteins and thus is toxic for cell growth. On the Escherichia coli genome, the nemA gene coding for NEM reductase is located downstream of the gene encoding an as-yet-uncharacterized transcription factor, YdhM. Disruption of the ydhM gene results in reduction of nemA expression even in the induced state, indicating that the two genes form a single operon. After in vitro genomic SELEX screening, ... |
| DOI | 10.1128/JB.00459-08 |
| ISSN | 1098-5530 |
| PMID | 18567656 |