マエダ　ミチヒサ   Maeda Michihisa   前田　理久    所属   明治大学  農学部    職種   専任教授
言語種別	英語
発行・発表の年月	1995/02
形態種別	学術雑誌
標題	Multiple genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase in the Gram-positive polychlorinated biphenyl-degrading bacterium Rhodococcus erythropolis TA421,isolated from termite ecosystem
執筆形態	共著(筆頭者)
掲載誌名	Appl. Environ. Microbiol.
巻・号・頁	61(22),pp.549-555
著者・共著者	M. Maeda, S. -Y. Chung, E. Song and T. Kudo
概要	Rhodococcus erythropolis TA421 was isolated from a termite ecosystem and is able to degrade a wide range of polychlorinated biphenyl (PCB) congeners. Genetic and biochem. analyses of the PCB catabolic pathway of this organism revealed that there are 4 different bphC genes (bphC1, bphC2, bphC3, and bphC4) which encode 2,3-dihydroxybiphenyl dioxygenases. As detd. by Southern hybridization, none of the bphC genes exhibits homol. to any other bphC gene. BphC1, bphC2, and bphC4 encode enzymes that have narrow substrate specificities and cleave the first arom. ring in the meta position. In contrast, bphC3 encodes a meta cleavage dioxygenase with broad substrate specificity. J. A. Asturias et al. (1994) have shown that the closely related organism Rhodococcus globerulus P6 contains 3 different bphC genes (bphC1, bphC2, and bphC3) which encode meta cleavage dioxygenases. The data suggest that there is a diverse family of bphC genes which encode PCB meta cleavage dioxygenases in members of the genus Rhodococcus.