ISHIMARU YOSHIRO
Department Undergraduate School , School of Agriculture Position Professor |
|
Research Period | 2008~2009 |
Research Topic | The in vivo roles of PKD1L3 and PKD2L1 in sour taste detection |
Research Type | KAKENHI Research |
Consignor | Japan Society for the Promotion of Science |
Research Program Type | Grant-in-Aid for Young Scientists (B) |
KAKENHI Grant No. | 20780089 |
Responsibility | Representative Researcher |
Representative Person | ISHIMARU Yoshiro |
Details | We identified the N-terminal extracellular region of human PKD1L3 and the full-length of human PKD2L1. HEK 293T cells expressing human PKD2L1 and chimeric PKD1L3, which consists of the N-terminal extracellular region of human PKD1L3 and the following region of mouse PKD1L3, showed off-response upon stimulation with sour solution such as citric acid. These cells still showed robust response to sour stimuli in the presence of a taste-modifying protein, neoculin, extracted from Curculigo latifolia. These results raise the possibility that neoculin inhibits the region other than the N-terminal extracellular region of human PKD1L3. Alternatively, inhibition event occurs at the level of taste cells or taste neurons, not at the taste receptors. |