OHSATO Shuichi
Department Undergraduate School , School of Agriculture Position Associate Professor |
|
Research Period | 2016/04~2019/03 |
Research Topic | A new technology for creating plant virus resistance by protein-protein interaction of RNA replicase and genome editing of host factor gene |
Research Type | KAKENHI Research |
Consignor | Japan Society for the Promotion of Science |
Research Program Type | Grant-in-Aid for Scientific Research (C) |
KAKENHI Grant No. | 16K07623 |
Responsibility | Research Contributor |
Representative Person | Kuwata Shigeru |
Details | To develop a new technology for creating plant virus resistance, we searched for peptides that bind to catalytic domains of RNA replicase and created plants that have deletions of a host factor for viral multiplication. We isolated 16 different peptides that bind to methyltransferase, helicase and polymerase domains of cucumber mosaic virus RNA replicase. In addition, genome editing was performed to create tobacco plants with a functional destruction of the tobacco eIF4E1 gene that is required for potato Y virus (PVY) multiplication. The tobacco plants showed strong resistance to PVY, demonstrating that conferring resistance to the virus can be achieved by destroying the host factor gene required by the virus. |