OHSATO Shuichi    Department   Undergraduate School  , School of Agriculture    Position   Associate Professor
Research Period	2016/04～2019/03
Research Topic	A new technology for creating plant virus resistance by protein-protein interaction of RNA replicase and genome editing of host factor gene
Research Type	KAKENHI Research
Consignor	Japan Society for the Promotion of Science
Research Program Type	Grant-in-Aid for Scientific Research (C)
KAKENHI Grant No.	16K07623
Responsibility	Research Contributor
Representative Person	Kuwata Shigeru
Details	To develop a new technology for creating plant virus resistance, we searched for peptides that bind to catalytic domains of RNA replicase and created plants that have deletions of a host factor for viral multiplication. We isolated 16 different peptides that bind to methyltransferase, helicase and polymerase domains of cucumber mosaic virus RNA replicase. In addition, genome editing was performed to create tobacco plants with a functional destruction of the tobacco eIF4E1 gene that is required for potato Y virus (PVY) multiplication. The tobacco plants showed strong resistance to PVY, demonstrating that conferring resistance to the virus can be achieved by destroying the host factor gene required by the virus.