シマダ　トモヒロ   SHIMADA TOMOHIRO   島田　友裕    所属   明治大学  農学部    職種   専任准教授
言語種別	英語
発行・発表の年月	2022/04
形態種別	学術雑誌
標題	Regulatory role of CsuR (YiaU) in determination of cell surface properties of Escherichia coli K-12.
執筆形態	共著(筆頭者)
掲載誌名	Microbiology (Reading, England)
掲載区分	国外
巻・号・頁	168(4)
担当区分	責任著者
著者・共著者	Tomohiro Shimada, Rie Murayama, Tomoki Mashima, Natsuko Kawano, Akira Ishihama
概要	Genomic SELEX screening was performed to identify the binding sites of YiaU, an uncharacterized LysR family transcription factor, on the Escherichia coli K-12 genome. Five high-affinity binding targets of YiaU were identified, all of which were involved in the structures of the bacterial cell surface such as outer and inner membrane proteins, and lipopolysaccharides. Detailed in vitro and in vivo analyses suggest that YiaU activates these target genes. To gain insight into the effects of YiaU in vivo on physiological properties, we used phenotype microarrays, biofilm screening assays and the sensitivity against serum complement analysed using a yiaU deletion mutant or YiaU expression strain. Together, these results suggest that the YiaU regulon confers resistance to some antibiotics, and increases biofilm formation and complement sensitivity. We propose renaming YiaU as CsuR (regulator of cell surface).
DOI	10.1099/mic.0.001166
PMID	35438626