サトウ　バン   SATO BAN   佐藤　伴    所属   明治大学  農学部    職種   助教
言語種別	日本語
発行・発表の年月	2020/10
形態種別	学術雑誌
査読	査読あり
標題	USP15 Deubiquitinates TUT1 Associated with RNA Metabolism and Maintains Cerebellar Homeostasis.
執筆形態	共著(筆頭者以外)
掲載誌名	Molecular and cellular biology
掲載区分	国外
巻・号・頁	40(21)
著者・共著者	Kim Jaehyun, Nakamura Junnosuke, Hamada Chiharu, Taketomi Takumi, Yano Sarasa, Okajima Tomomi, Kashiwabara Shin-Ichi, Baba Tadashi, Sato Ban, Chiba Tomoki, Tsuruta Fuminori
概要	Precise regulation of RNA metabolism is crucial for dynamic gene expression and controlling cellular functions. In the nervous system, defects in RNA metabolism are implicated in the disturbance of brain homeostasis and development. Here, we report that deubiquitinating enzyme, ubiquitin specific peptidase 15 (USP15), deubiquitinates terminal uridylyl transferase 1 (TUT1) and changes global RNA metabolism. We found that the expression of USP15 redistributes TUT1 from the nucleolus to nucleoplasm, resulting in the stabilization of U6 snRNA. We also found that lack of the Usp15 gene induces an impairment in motor ability with an unconventional cerebellar formation. Therefore, our results suggest that USP15 is crucial for mRNA metabolism and maintains a healthy brain. These findings provide a possibility that disturbance of the USP15-TUT1 cascade induces chronic and mild ER stress, leading to an acceleration of the neurodegenerative phenotype.
DOI	10.1128/MCB.00098-20
ISSN	1098-5549
PMID	32839293