イヌイ　マサフミ   INUI MASAFUMI   乾　雅史    所属   明治大学  農学部    職種   専任教授
言語種別	英語
発行・発表の年月	2020/05
形態種別	学術雑誌
査読	査読あり
標題	Lin28a/let-7 pathway modulates the Hox code via Polycomb regulation during axial patterning in vertebrates.
執筆形態	共著(筆頭者以外)
掲載誌名	eLife
掲載区分	国外
巻・号・頁	9
著者・共著者	Sato Tempei, Kataoka Kensuke, Ito Yoshiaki, Yokoyama Shigetoshi, Inui Masafumi, Mori Masaki, Takahashi Satoru, Akita Keiichi, Takada Shuji, Ueno-Kudoh Hiroe, Asahara Hiroshi
概要	The body plan along the anteroposterior axis and regional identities are specified by the spatiotemporal expression of Hox genes. Multistep controls are required for their unique expression patterns; however, the molecular mechanisms behind the tight control of Hox genes are not fully understood. In this study, we demonstrated that the Lin28a/let-7 pathway is critical for axial elongation. Lin28a-/- mice exhibited axial shortening with mild skeletal transformations of vertebrae, which were consistent with results in mice with tail bud-specific mutants of Lin28a. The accumulation of let-7 in Lin28a-/- mice resulted in the reduction of PRC1 occupancy at the Hox cluster loci by targeting Cbx2. Consistently, Lin28a loss in embryonic stem-like cells led to aberrant induction of posterior Hox genes, which was rescued by the knockdown of let-7. These results suggest that the Lin28/let-7 pathway is involved in the modulation of the 'Hox code' via Polycomb regulation during axial patterning.
DOI	10.7554/eLife.53608
ISSN	2050-084X
PMID	32479258