サトウ　バン   SATO BAN   佐藤　伴    所属   明治大学  農学部    職種   助教
言語種別	日本語
発行・発表の年月	2007/12
形態種別	学術雑誌
査読	査読あり
標題	Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos.
執筆形態	共著(筆頭者以外)
掲載誌名	Biochemical and biophysical research communications
掲載区分	国外
巻・号・頁	364(4),838-43頁
著者・共著者	Sato Ban, Katagiri Yohko U, Miyado Kenji, Akutsu Hidenori, Miyagawa Yoshitaka, Horiuchi Yasuomi, Nakajima Hideki, Okita Hajime, Umezawa Akihiro, Hata Jun-Ichi, Fujimoto Junichiro, Toshimori Kiyotaka, Kiyokawa Nobutaka
概要	The monoclonal antibody 6E2 raised against the embryonal carcinoma cell line NCR-G3 had been shown to also react with human germ cells. Thin-layer chromatography (TLC) immunostaining revealed that 6E2 specifically reacts with sialosylglobopenta osylceramide (sialylGb5), which carries an epitope of stage-specific embryonic antigen-4 (SSEA-4), known as an important cell surface marker of embryogenesis. The immunostaining of mouse preimplantation embryos without fixation showed that the binding of 6E2 caused the clustering and consequent accumulation of sialylGb5 at the interface between blastomeres. These results suggest that SSEA-4 actively moves on the cell surface and readily accumulates between blastomeres after binding of 6E2.
DOI	10.1016/j.bbrc.2007.10.093
ISSN	1090-2104
PMID	18273440