シマダ　トモヒロ   SHIMADA TOMOHIRO   島田　友裕    所属   明治大学  農学部    職種   専任准教授
言語種別	日本語
発行・発表の年月	2018
形態種別	著書
標題	Genomic SELEX Screening of Regulatory Targets of Escherichia coli Transcription Factors.
執筆形態	共著(筆頭者)
掲載誌名	Methods in molecular biology (Clifton, N.J.)
掲載区分	国外
巻・号・頁	1837,49-69頁
著者・共著者	Shimada Tomohiro, Ogasawara Hiroshi, Ishihama Akira
概要	The genome of Escherichia coli K-12 is transcribed by a single species of RNA polymerase. The selectivity of its transcriptional targets is modulated via two-steps of protein-protein interaction: at the first step, seven species of the sigma subunit are involved, at the second step, a total of approximately 300 species of transcription factor (TFs). For the identification of the regulatory targets of these two groups of regulatory proteins, we developed two in vitro approaches, "Genomic SELEX" (currently designated as gSELEX) and "PS (promoter-specific)-TF" screenings. Here, we describe a detailed protocol of the genomic SELEX screening system which uses purified regulatory proteins and fragments of genomic DNA from E. coli.
DOI	10.1007/978-1-4939-8675-0_4
ISSN	1940-6029
PMID	30109605