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シマダ トモヒロ
SHIMADA TOMOHIRO
島田 友裕 所属 明治大学 農学部 職種 専任准教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2014/07 |
| 形態種別 | 学術雑誌 |
| 査読 | 査読あり |
| 標題 | Unprecedented high-resolution view of bacterial operon architecture revealed by RNA sequencing. |
| 執筆形態 | 共著(筆頭者以外) |
| 掲載誌名 | mBio |
| 掲載区分 | 国外 |
| 巻・号・頁 | 5(4),pp.e01442-14 |
| 国際共著 | 国際共著 |
| 著者・共著者 | Conway Tyrrell, Creecy James P, Maddox Scott M, Grissom Joe E, Conkle Trevor L, Shadid Tyler M, Teramoto Jun, San Miguel Phillip, Shimada Tomohiro, Ishihama Akira, Mori Hirotada, Wanner Barry L |
| 概要 | We analyzed the transcriptome of Escherichia coli K-12 by strand-specific RNA sequencing at single-nucleotide resolution during steady-state (logarithmic-phase) growth and upon entry into stationary phase in glucose minimal medium. To generate high-resolution transcriptome maps, we developed an organizational schema which showed that in practice only three features are required to define operon architecture: the promoter, terminator, and deep RNA sequence read coverage. We precisely annotated 2,122 promoters and 1,774 terminators, defining 1,510 operons with an average of 1.98 genes per operon. ... |
| DOI | 10.1128/mBio.01442-14 |
| ISSN | 2150-7511 |
| PMID | 25006232 |