シマダ　トモヒロ   SHIMADA TOMOHIRO   島田　友裕    所属   明治大学  農学部    職種   専任准教授
言語種別	英語
発行・発表の年月	2017
形態種別	学術雑誌
査読	査読あり
標題	The whole set of the constitutive promoters recognized by four minor sigma subunits of Escherichia coli RNA polymerase.
執筆形態	共著(筆頭者)
掲載誌名	PloS one
掲載区分	国外
巻・号・頁	12(6),pp.e0179181
担当区分	筆頭著者
著者・共著者	Shimada Tomohiro, Tanaka Kan, Ishihama Akira
概要	The promoter selectivity of Escherichia coli RNA polymerase (RNAP) is determined by the sigma subunit. The model prokaryote Escherichia coli K-12 contains seven species of the sigma subunit, each recognizing a specific set of promoters. For identification of the "constitutive promoters" that are recognized by each RNAP holoenzyme alone in the absence of other supporting factors, we have performed the genomic SELEX screening in vitro for their binding sites along the E. coli K-12 W3110 genome using each of the reconstituted RNAP holoenzymes and a collection of genome DNA segments of E. coli K-12. ...
DOI	10.1371/journal.pone.0179181
ISSN	1932-6203
PMID	28666008