シマダ　トモヒロ   SHIMADA TOMOHIRO   島田　友裕    所属   明治大学  農学部    職種   専任准教授
言語種別	日本語
発行・発表の年月	2019/01
形態種別	学術雑誌
査読	査読あり
標題	Regulatory role of CsqR (YihW) in transcription of the genes for catabolism of the anionic sugar sulfoquinovose (SQ) in Escherichia coli K-12.
執筆形態	共著(筆頭者)
掲載誌名	Microbiology (Reading, England)
掲載区分	国外
巻・号・頁	165(1),78-89頁
著者・共著者	Shimada Tomohiro, Yamamoto Kaneyoshi, Nakano Masahiro, Watanabe Hiroki, Schleheck David, Ishihama Akira
概要	The binding sites of YihW, an uncharacterized DeoR-family transcription factor (TF) of Escherichia coli K-12, were identified using Genomic SELEX screening at two closely located sites, one inside the spacer between the bidirectional transcription units comprising the yihUTS operon and the yihV gene, and another one upstream of the yihW gene itself. Recently the YihUTS and YihV proteins were identified as catalysing the catabolism of sulfoquinovose (SQ), a hydrolysis product of sulfoquinovosyl diacylglycerol (SQDG) derived from plants and other photosynthetic organisms. Gel shift assay in vitro and reporter assay in vivo indicated that YihW functions as a repressor for all three transcription units. De-repression of the yih operons was found to be under the control of SQ as inducer, but not of lactose, glucose or galactose. Hence, as a regulator of the catabolism of SQ, we renamed YihW as CsqR.
DOI	10.1099/mic.0.000740
ISSN	1465-2080
PMID	30372406