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INUI MASAFUMI
Department Undergraduate School , School of Agriculture Position Associate Professor |
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| Language | English |
| Publication Date | 2020/05 |
| Type | Academic Journal |
| Peer Review | Peer reviewed |
| Title | Lin28a/let-7 pathway modulates the Hox code via Polycomb regulation during axial patterning in vertebrates. |
| Contribution Type | Co-authored (other than first author) |
| Journal | eLife |
| Journal Type | Another Country |
| Volume, Issue, Page | 9 |
| Author and coauthor | Sato Tempei, Kataoka Kensuke, Ito Yoshiaki, Yokoyama Shigetoshi, Inui Masafumi, Mori Masaki, Takahashi Satoru, Akita Keiichi, Takada Shuji, Ueno-Kudoh Hiroe, Asahara Hiroshi |
| Details | The body plan along the anteroposterior axis and regional identities are specified by the spatiotemporal expression of Hox genes. Multistep controls are required for their unique expression patterns; however, the molecular mechanisms behind the tight control of Hox genes are not fully understood. In this study, we demonstrated that the Lin28a/let-7 pathway is critical for axial elongation. Lin28a-/- mice exhibited axial shortening with mild skeletal transformations of vertebrae, which were consistent with results in mice with tail bud-specific mutants of Lin28a. The accumulation of let-7 in Lin28a-/- mice resulted in the reduction of PRC1 occupancy at the Hox cluster loci by targeting Cbx2. Consistently, Lin28a loss in embryonic stem-like cells led to aberrant induction of posterior Hox genes, which was rescued by the knockdown of let-7. These results suggest that the Lin28/let-7 pathway is involved in the modulation of the 'Hox code' via Polycomb regulation during axial patterning. |
| DOI | 10.7554/eLife.53608 |
| ISSN | 2050-084X |
| PMID | 32479258 |