KAWANO NATSUKO    Department   Undergraduate School  , School of Agriculture    Position   Associate Professor
Language	English
Publication Date	2018
Type	Academic Journal
Peer Review	Peer reviewed
Title	Ubiquitin-activating enzyme E1 inhibitor PYR-41 retards sperm enlargement after fusion to the egg.
Contribution Type	Co-authored (other than first author)
Journal	Reproductive Toxicology
Journal Type	Another Country
Volume, Issue, Page	76,pp.71-77
Author and coauthor	K. Yoshida, W. Kang, A. Nakamura, N. Kawano, M. Hanai, M. Miyado, Y. Miyamot, M. Iwai, T.Hamatani, H. Saito, K. Miyado, A. Umezawa
Details	The ubiquitin-proteasome system, which is initiated by a single ubiquitin-activating enzyme E1 (UBE1), is involved in male reproduction via spermatogenesis and function in mammals. Here we explored the influence of UBE1-specific inhibitor, 4[4-(5-nitro-furan-2-ylmethylene)-3,5-dioxo-pyrazolidin-1-yl]-benzoic acid ethyl ester (pyrazone-41 or PYR-41) in female reproduction. UBE-1 was detected by immunoblotting and immunocytochemistry in mouse eggs and was localized mainly under the egg plasma membrane. PYR-41 pretreatment suppresses the development of eggs into two-cell embryos. Specifically, pretreatment retarded sperm enlargement and meiotic chromosomal division after sperm-egg fusion. PYR-41 pretreatment disturbed β-catenin, a well-known target protein for ubiquitination, localization under the egg plasma membrane and on spindle microtubules in wild-type eggs. Otherwise, PYR-41 treatment had no effect on the two-cell development of eggs lacking β-catenin. Our results raise the possibility that inhibition of the ubiquitin-proteasome system suppresses sperm enlargement through impaired β-catenin-mediated mechanism.